Current research work describes rapid high performance thin layer chromatographic determination of artemether and lumefantrine form its combined pharmaceutical formulation. The mentioned drugs were spotted on silica gel F254 TLC plates under pure nitrogen stream by linomat TLC spotter. Separation was carried out by using chloroform, methanol, ethyl acetate and triethylamine as mobile phase in ratio of 4:2.5:3.5:0.1 v/v/v/v. Developed TLC plates were scanned by CAMAG TLC scanner and detection was carried out at 235 nm. Rf value of separated drugs was found to be 0.48 and 0.78 for artemether and nlumefantrine respectively. The developed method was validated as per ICH guidelines by studying various validation parameters like accuracy, precision, robustness, LOD, LOQ and solvent stability. The developed and validated method was successfully applied for determination of artemether and lumefantrine from its combined pharmaceutical formulation.
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